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SRX275213: Survey of transcriptomes Brevibacillus borstelensis in response to low and high temperature shock
1 ILLUMINA (Illumina HiSeq 2000) run: 13.1M spots, 2.6G bases, 1.7Gb downloads

Design: Bacterial culture of Brevibacillus borstelensis was grown in NB media at 50 degree Celsius and RNA was isolated from cells. Library preparation was started with mRNA fragmentation followed by reverse transcription, second-strand synthesis, pair-end adapter ligation, and finally ended by amplification of adaptor-ligated library. Library quantification and qualification was performed on Agilent bioanalyzer using High Sensitivity DNA Chip. The library of sample came into size range of 250 bp to 400 bp.
Submitted by: DIVISION OF FISH HEALTH MANAGEMENT, CENTRAL INSTITUTE OF FRESHWATER AQUACULTURE BHUBANESWAR-751002, ORISSA. INDIA
Study: Brevibacillus borstelensis Transcriptome or Gene expression
show Abstracthide Abstract
To identify and understand the role of stress-relevant genes responsible for the survival mechanism of the thermophiles in a wide range of environment, whole transcriptome analysis of Brevibacillus borstelensis was performed de novo by Illumina Hi Seq 2000 sequencing technology. The gene expression profile of B. borstelensis was analyzed after the exposure of the bacterium to low temperature treatment (20°C for 30 min) and high temperature treatment (55°C for 5 min and 10 min). The total number of genes that revealed significant similarity to known functional protein-coding genes were annotated using the GO and KEGG pathway databases. Differential profiling of the genes expressed in the control (50°C) and treated samples revealed the significantly up regulated and down regulated genes. This brief survey of the transcripts obtained in response to low and high temperature shock underline the survival strategy of a thermophilic bacterium under different temperature parameters that is further helpful to generate vast genetic information associated with the bacteria.
Sample: Generic sample from Brevibacillus borstelensis
SAMN02086201 • SRS418617 • All experiments • All runs
Library:
Name: Bb_50
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward102  reverse

Runs: 1 run, 13.1M spots, 2.6G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR85182513,072,1672.6G1.7Gb2013-06-12

ID:
389956

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